Membranes for Lateral Flow Tests

Nupore produces highly consistent and optimized Nitrocellulose (NC) membranes for lateral flow tests. A wide variety of membranes are available in roll form as standard products to meet various needs.

Types Available

Membranes are directly cast on a transparent polyester backing to improve the handling strength. Standard polyester film is 100 µm thick, although other thickness can be available on order.

Specifications :

Type LFNC - C Lower protein binding
Type LFNC - H Higher protein binding
Type LFNC - F Highest protein binding

 

Technical Data

The wicking rate is an important characteristic of NC membrane for lateral flow tests and primarily determines the reaction kinetics. Nupore membranes are designated by pore size/wicking rate and have reproducible and defined wicking rates. The wicking rate of blocked membranes will, however, depend on the blocking protocol.

Ordering Information :

Type Rating (µm) Membrane Thickness (µm) 4cm Wicking Time
(Sec)
LFNC-C 8 100 160
10 100 130
12 100 100
15 100 80
LFNC - H 12 100 100
15 100 80
LFNC - F 90 100 90
130 100 130
150 100 150
170 100 170

 

Storage

Nupore recommends storage of membrane rolls at 15°C to 20°C for longer shelf life. The membrane rolls should be kept away from heat radiators. The membrane is inflammable when subjected to direct heat.

How to select

Selection of membrane depends on the assay. Highest sensitivity is obtained with smaller pore size (slower wicking rate). For high affinity Ag/Ab reaction, use of bigger pore size (faster wicking rates) can result in faster test with adequate sensitivity.

Blocking of Membranes

Although wet blocking of membrane (dipping the membrane in solution and drying) is cumbersome and adds cost to the production process, it is sometimes necessary to achieve important assay requirements such as stabilizing the Ab, reducing non-specific reaction, improving the wicking front, and migration of particles etc. Many excellent assays, however, can be made without wet blocking the membrane. All Nupore membranes exhibit extremely stable wicking rates with time without blocking and can be used successfully for making assays without blocking.